fluorescence intensity by ivis  (Caliper Life Sciences)

Journal: Scientific Reports

Article Title: Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

doi: 10.1038/s41598-025-31872-8

Figure Lengend Snippet: ( A ). Representative images of OE-19 xenograft bearing mice imaged at 4 and 6 h post NF-800 injection at IVIS Spectrum. ( B ) Mean fluorescence intensity values of Tumor, Kidney and Liver of animals bearing OE-19 xenografts and treated with NF-800. ( C ) Lifetime values obtained with tail fitting of the abovementioned tissues, compared to the probe dissolved in PBS (n = 7 animals). One way ANOVA Multiple comparison test (* = p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001).

Article Snippet: Fluorescence intensity acquisitions were performed at IVIS Spectrum (Perkin Elmer Italia S.p.A.) equipped with an excitation filter at 745 nm and emission filter at 800 nm with 30 nm and 20 nm bandwidth, respectively.

Techniques: Injection, Fluorescence, Comparison

Journal: Scientific Reports

Article Title: Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

doi: 10.1038/s41598-025-31872-8

Figure Lengend Snippet: ( A ) Fluorescence intensity values (counts) and respective lifetimes (ns) of ex vivo tumor and kidneys 6 h after administration of NF-800. ( B ) Fluorescence intensity values and respective lifetimes (ns) of ex vivo tumor and liver after administration of Tz-800. (One way ANOVA Multiple comparison test was performed between all the groups (e.g. tumor vs kidney; tumor vs PBS; kidney vs PBS) (* = p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001).

Article Snippet: Fluorescence intensity acquisitions were performed at IVIS Spectrum (Perkin Elmer Italia S.p.A.) equipped with an excitation filter at 745 nm and emission filter at 800 nm with 30 nm and 20 nm bandwidth, respectively.

Techniques: Fluorescence, Ex Vivo, Comparison

Journal: Scientific Reports

Article Title: Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

doi: 10.1038/s41598-025-31872-8

Figure Lengend Snippet: Representative Fluorescence Intensity (AU) and FLT parametric map (ns) of a tumor treated with NF-800. ROIs indicate tumors, which appears to have spread throughout the stomach. Histogram represents distribution of lifetimes across pixels, as fitted to obtain the parametric map.

Article Snippet: Fluorescence intensity acquisitions were performed at IVIS Spectrum (Perkin Elmer Italia S.p.A.) equipped with an excitation filter at 745 nm and emission filter at 800 nm with 30 nm and 20 nm bandwidth, respectively.

Techniques: Fluorescence

Journal: Nano Convergence

Article Title: Tumor-specific biochemical nanoconversion of self-assembled peptide-conjugated paclitaxel-docetaxel-based nanoparticles

doi: 10.1186/s40580-025-00487-0

Figure Lengend Snippet: In vitro, cathepsin B cleavage, cellular toxicity and fluorescence imaging of (Ac)FRRF-DTX NPs. HPLC analysis of (Ac)FRRF-DTX NPs incubated without ( a ) or with ( b ) enzyme reaction buffer containing cathepsin B for different time points (0–72 h). ( c ) LC-MS analysis of PTXm during the cathepsin B cleavage experiment. ( d ) The assessment of cathepsin B activity levels in HDFa, Hep G2, CT26.wt, and A549 cell lines ( n = 3). ( e ) Evaluation of anticancer efficacy on Hep G2 cells treated with DTX, PTX, PTXm, (Ac)FRRF, (Ac)FRRF-DTX NPs and de-Boc-DTX ( n = 5 − 6). ( f ) Cellular uptake of RITC-PTX and RITC-(Ac)FRRF-DTX NPs in Hep G2 cells (Scale bar = 50 μm). ( g ) Data quantifying the fluorescence intensity over time of (Ac)FRRF-DTX NPs and PTX ( n = 3). ( h ) Fluorescent microscopy imaging observing cell death in Hep G2 cells treated with PTX, PTXm and (Ac)FRRF-DTX NPs (Scale bar = 50 μm). ( i ) Quantification of fluorescence intensity from nucleic acid staining using ImageJ software ( n = 5). ( j ) microtubule binding of PTX, PTXm and (Ac)FRRF-DTX NPs (Scale bar = 50 μm). ( k ) Quantification of fluorescence intensity from Microtubule Cytoskeleton Dye using ImageJ software ( n = 5). ( l ) Confocal images of (Ac)FRRF-DTX NPs (Scale bar = 10 μm). *p < 0.05, **p < 0.01 and ***p < 0.001

Article Snippet: The fluorescence intensity of RITC-(Ac)FRRF-DTX was measured and quantified using a precision fluorescence analyzer (FOBI, CELLGENTEK, Republic of Korea) at various time points after intravenous injection (0, 0.5, 1, 3, 6, 12, 24 and 48 h).

Techniques: In Vitro, Fluorescence, Imaging, Incubation, Liquid Chromatography with Mass Spectroscopy, Activity Assay, Microscopy, Staining, Software, Binding Assay

Journal: Nano Convergence

Article Title: Tumor-specific biochemical nanoconversion of self-assembled peptide-conjugated paclitaxel-docetaxel-based nanoparticles

doi: 10.1186/s40580-025-00487-0

Figure Lengend Snippet: In vivo biodistribution and retention level in the bloodstream of (Ac)FRRF-DTX NPs. ( a ) Scheme describing the in vivo distribution study of (Ac)FRRF-DTX NPs using BALB/c nude mice. ( b ) In vivo distribution over time of RITC-(Ac)FRRF-DTX NPs administered via tail vein injection in BALB/c nude mice. ( c ) Quantified graphs depicting fluorescent intensity in tumor regions ( n = 3). Images showing the measurement of fluorescence intensity from hourly extractions of organs and tumors in mice treated with RITC-(Ac)FRRF-DTX NPs ( d ) and treated with RITC-de-Boc-DTX ( e ). ( f ) Quantified graphs depicting fluorescent intensity in tumor tissues over time ( n = 3). ( g ) The concentration in the bloodstream of Sprague-Dawley rats treated with RITC-(Ac)FRRF-DTX NPs and RITC-de-Boc-DTX administered via tail vein injection ( n = 3). The AUC (h) and half-life (i) ( n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001

Article Snippet: The fluorescence intensity of RITC-(Ac)FRRF-DTX was measured and quantified using a precision fluorescence analyzer (FOBI, CELLGENTEK, Republic of Korea) at various time points after intravenous injection (0, 0.5, 1, 3, 6, 12, 24 and 48 h).

Techniques: In Vivo, Injection, Fluorescence, Concentration Assay

Journal: Cell death discovery

Article Title: RBM47 inhibits hepatocellular carcinoma progression by targeting UPF1 as a DNA/RNA regulator.

doi: 10.1038/s41420-022-01112-3

Figure Lengend Snippet: Fig. 2 RBM47 inhibits tumor growth and metastasis in vivo. A Representative images of removed subcutaneously implanted tumors. B The effects of RBM47 on tumor growth are summarized by a tumor growth curve. Error bars are SD (n = 5). C Comparison of tumor weight between the RBM47 overexpression group and the control group. Error bars are SD (n = 5). Microscopic images of HE (D) and immunohistochemical staining patterns for Ki-67 (E) in tumor xenografts from the nude mice in the RBM47 overexpression group and the control group (scale bars = 50 μm). F Observation of lung metastasis by in vivo imaging after tail vein injection with cells stably transfected with RBM47 or empty vector with fluorescence labeling (left panel) and quantitative analysis of fluorescence intensity (right panel). Error bars are SD (n = 5). **P < 0.01, ***P < 0.001.

Article Snippet: At the end of the 5th week, the lung fluorescence intensities in vivo were tested by an IVIS® Lumina III System (PerkinElmer, USA) to quantitatively compare metastasis.

Techniques: In Vivo, Comparison, Over Expression, Control, Immunohistochemical staining, Staining, In Vivo Imaging, Injection, Stable Transfection, Transfection, Plasmid Preparation, Labeling

Journal: PLoS ONE

Article Title: Quantitative Evaluation of the Reticuloendothelial System Function with Dynamic MRI

doi: 10.1371/journal.pone.0103576

Figure Lengend Snippet: The blue dots on Prussian blue staining are the liver-accumulated SPIO nanoparticles; the green fluorescence on fluorescence images is macrophage. d. The area occupied by kupffer cells and SPIO nanoparticles estimated by imaging analysis in liver and spleen. (**p<0.005 compared with saline group. n = 4).

Article Snippet: The major organs including the liver, lung, spleen, kidney, brain, and heart were dissected from the mice, and their fluorescence intensities were determined using a IVIS Spectrum Pre-clinical In Vivo Imaging System (PerkinElmer, USA) equipped with a QD800 band pass filter (excitation/emission 675/800 nm).

Techniques: Staining, Fluorescence, Imaging, Saline